Journal: Journal of Nuclear Medicine
Article Title: Establishing 177 Lu-PSMA-617 Radioligand Therapy in a Syngeneic Model of Murine Prostate Cancer
doi: 10.2967/jnumed.117.193359
Figure Lengend Snippet: PSMA expression of mouse RM1 versus human C4-2 prostate cancer cell lines. (A) PSMA expression was determined in vitro by flow cytometry for RM1-hPSMA (green), RM1-PGLS (blue), and C4-2 (red). RM1-PGLS cells exhibit heterogeneous PSMA expression, with PSMA-low (light blue) and PSMA-high (dark blue) subpopulations. (B) Mean absolute numbers of PSMA on cell surface were quantified on basis of mean fluorescence intensity values using antihuman IgG quantification beads. (C) Representative 68Ga-PSMA-11 PET/CT maximum-intensity projection of mice bearing equally sized subcutaneous mouse allografts or human xenografts. Mean %IA/g was 22.7, 17.4, and 15.5 for RM1-hPSMA, RM1-PGLS, and C4-2, respectively (n = 2 each).
Article Snippet: Absolute numbers of PSMA proteins on the cell surface were quantified by flow cytometry based on mean fluorescence intensity values using the Quantum Simply Cellular (Bangs Laboratory) antihuman IgG quantification beads according to the manufacturer’s instructions.
Techniques: Expressing, In Vitro, Flow Cytometry, Fluorescence, Positron Emission Tomography-Computed Tomography